Fig. (16) Oil red O staining of hMSC-TERT after adipogenic cultivation for 16 days. The cells were cultured in spinner flasks and harvested by means of accutase (a), trypsin (b) and a 1:1 (w/w) trypsin accutase mix (c) before adipogenic cultivation in 6-well cell culture plates. hMSC-TERT cultured in tissue culture flasks and harvested with the trypsin-accutase mix shown as reference (d). Negative controls without adipogenic stimulation were performed with cells, which were grown in a spinner (e) and tissue culture flask (f) and harvested using the trypsin-accutase mixture.
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