Table 1: Metal Ion Binding (MIB) Server [33] results showing the different binding affinities and the different interacting residues per cofactor-bound S100β protein with the cofactors represented as spherical balls.

Cofactor-Bond S100β Binding Residues Binding Scores 3D Model of the Best Binding Mode
Ca 2+ - S100β 62D, 64D, 66D, 68E, 73E 2.891
62D, 64D, 65G, 66D, 68E, 69C, 73E 2.766
Mg2+ - S100β 62D, 64D, 66D, 68E 2.031
62D, 64D, 66D, 68E, 73E 1.944
Zn2+ - S100β 86H, 87E 1.538
55D, 59E 1.480