Fig. (4) Biocompatibility and delayed adherence of cultured cells on madurahydroxylactone coatings. NIH3T3 cells (A and C) and HEK 293 cells (B and D) were seeded on glass microscope cover slips coated with a 1:1 (w/w) mixture of madurahydroxylactone with ciprofloxacin (A and B) or without coating (C and D). The cells were cultured in DMEM with 10% FCS under standard conditions and microphotography images were taken after 1, 2 or 4 days as indicated. The scale bar corresponds to 100 µm. The white arrows point out red stained cells. HEK 293 cell density after incubation for the time indicated on the following surfaces: CCP (black bars), glass (gray bars), MHL (empty bars) (E).