Fig. (2) Ion exchange chromatography and SDS-PAGE of L-asparaginases. The sonicated crude extract was chromatographed on DEAE-Sepharose. Total protein was monitored at 280 nm. L-asparaginase was purified from Bacillus PG03 (A) and Bacillus PG04 (B). Dashed line represents OD 280 nm, Solid line represents enzyme activity. (C) SDS-PAGE of the purified L-asparaginases. Molecular weight marker (lane 1), L-asparaginase from Bacillus PG03 (lane 2) and Bacillus PG04 (lane 3).