Fig. (3) (A) Effect of pH and (B) temperature on activity of L- asparaginase from Bacillus PG03 (closed diamonds) and Bacillus PG04 (open circles). (C) Lineweaver–Burk plots of L-asparaginase activity purified from Bacillus PG03 (closed diamonds) and Bacillus PG04 (open circles).