Protease specificity determination using model proteases and CNBr cleavage
Mass spectra of trypsin, V8 protease, elastase and cyanogen bromide digestion. A: The fragments detected after cleavage of C-terminal Lys and Arg with tryptic digestion. B: The fragments generated from the cleavage of C-terminal Glu and Asp with V8 protease digestion. C: The fragments cleaved from the C-terminal after Ala and Val by elastase. D: The fragments generated from the cleavage of C-terminal Met after CNBr digestion. The mass spectra detected homoserine lactone converted from methionine during CNBr digestion. E: The biotin-labeled substrates treated with inactivated trypsin, which was boiled for 10 min, as a negative control.
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