Fig. (1) Effect of DHA on the proliferation of VSMCs induced by IHG. Quiescent cultures of VSMC were pretreated with DHA and exposed to constant or intermittent high glucose level for 72 hrs. After treatment, cell survival was examined using the MTT reduction assay. Cell viability was calculated as the percentage of the control group. The data represent mean ± SEM of 3 individual experiments. *P < 0.001 versus Con, # P < 0.001 versus SHG, ** P < 0.001 versus IHG.