Effects of Triton X-100 and PEG on the Catalytic Properties and Thermal Stability of Lipase from Candida Rugosa Free and Immobilized on Glyoxyl-Agarose

Fig. (3) Thermal stability at 45 ºC and pH 8.0 for CRL free and immobilized on glyoxyl-agarose (absence and presence of Triton-X-100 and PEG). Half-lives estimated by Sadana-Henley model [57]: free CRL (t_1/2 = 18.5 min); CRL-glyoxyl (t_1/2 = 34.5 min); CRL-glyoxyl, presence of Triton-X-100 (t_1/2 = 19.0 min); CRL-glyoxyl, presence of PEG (t_1/2 = 109 min).