^a Enzyme load offered as protein mass per support mass, about 1 mg g^-1 and reaction time of 2h. ^b IE – Immobilized enzyme (derivative CRL-agarose). ^c Enzyme load offered as protein per support mass, about 5 mg g^-1 and reaction time of 4h. ^d Activity recovery was defined as the ratio of the immobilized enzyme activity and the enzyme activity offered for immobilization per gram of support. Results are expressed as average of triplicate assays (standard deviations less than 10%). Activities were measured at 37^oC and pH 7.0 using olive oil as substrate (Section 2.3 – Activity assay).