Supplement-Table 1:
Dnase-1 mix for samples.
V= 30 μL
1.
10Хbuffer (for Taq- polymerase, with 25 mM MgCl
2
and 1mM CaCl
2
)
3 μL
2.
RNA
23.3 μL (1μg)
3.
1u/μL DNAse-1 (RNase free)
3 μL
4.
40 u/ μL RNasin
0.7 μL