Supplement-Table 3:
Real-time PCR mix for samples.
V= 25μL
1.
10Хbuffer (for Taq- polymerase with SybrGreen))
2.5 μL
2.
2.5 mM dNTPs
2.5 μL
3.
25 mM MgCl
2
2.5 μL
4.
10 μM Primers
per1.0 μL
6.
cDNA
5 μL
7.
Н
2
О
Q
10 μL
8.
5000 u/ml Taq_ polymerase
0.25 μL