Fig. (2) Interactions between the eL42 species and the translation termination factor eRF1. (A), the His-tagged recombinant human eL42 was immobilized on the surface of CM5 sensor chip at low density resonance units (RU). Various concentrations of eRF1 (0, 0.2, 0.3, 0.6 and 1 μM) were run over the chip surface. (B), the same experiment as in (A), with the His-tagged human eL42-Δ(GGQTK) mutant, the eL42 protein lacking the 49GGQTK53 pentapeptide. The concentrations of eRF1 used were: 0, 0.1, 0.2, 0.25, 0.3, 0.35 and 0.4 μM. (C), eL42-Q51E mutant in the presence of eRF1 at the concentrations of 0, 0.04, 0.06, 0.08, 0.1, 0.12 and 0.14 μM. (D), eL42-K53Q mutant in the presence of eRF1 at the concentrations of 0, 0.04, 0.08, 0.12, 0.16, 0.2, 0.24 and 0.28 μM. (E), eL42-Q51A/K53A mutant in the presence of eRF1 at the concentrations of 0, 0.02, 0.05, 0.06, 0.07, 0.08, 0.1 and 0.12 μM.