Fig. (4) Interactions between human eL42 and the yeast elongation factor 3 (EF-3). (A), His-tagged human eL42 immobilized on CM5 sensor chip. Various concentrations of S. cerevisiae EF-3 (0, 0.1, 0.15, 0.25, 0.3, 0.35, 0.4 and 0.45 μM) were run over the chip surface. (B), the same experiment as in (A), with the His-tagged human eL42-Δ(GGQTK) mutant and with the EF-3 concentrations of 0, 0.1, 0.2, 0.3, 0.4 and 0.45 μM.