Fig. (5) Control experiments of the interactions between eL42 and the translation factors. (A), positive control consisting of the measurement of the interaction between eL42 and the anti-eL42 antibodies. (B), negative control showing the absence of interaction between eL42 and tRNA nucleotidyl transferase (CCAse), in comparison with the translation factors eRF1 and EF-3. (C), the same experiment as in (B), with the eL42-Δ(GGQTK) variant. (D), negative control showing the absence of interaction between the eL42-Q51A/K53A mutant and CCAse, in comparison with the translation factors eRF1 and EF-3.