Fig. (6) Kinetic measurement of eL42:tRNA or eL42:rRNA interactions. (A), His-tagged human eL42 was immobilized on the surface of NTA sensor chip at low density resonance units (RU). Various concentrations of tRNA-Asp (0, 1, 2, 4, 5 and 6 nM) were run over the chip surface. (B), the same experiment as in (A), with the His-tagged eL42-Δ(GGQTK) mutant and with tRNA-Asp concentrations of 0, 1, 2, 4, 5 and 6 nM. (C), interactions between His-tagged eL42 and rRNA at the concentrations of 0, 0.02, 0.040, 0.060, 0.080, 0.1, 0.12, 0.140, 0.160, 0.180 and 0.200 nM. (D), the same experiment as in (C), with the His-tagged eL42-Δ(GGQTK) mutant and with rRNA at the concentrations of 0, 0.05, 0.1, 0.2, 0.3, 0.4, and 0.5 nM. (E), His-tagged human eL42 and eRF1 were each immobilized on the surface of NTA sensor chip, and tRNA (20 nM) was run over the chip surface. (F), the same experiment as in (E), with rRNA (10 nM) instead of tRNA.