Table 1:
Steady-state kinetic parameters of HsFH, A308T, and H318Y.
Enzyme
V
max
(µmole /min/mg)
k
cat
(s
-1
)
K
m
(mM)
k
cat
/K
m
(M
-1
s
-1
)
HsFH
170 ± 10
150 ± 9
1.9 ± 0.2
8 x 10
4
± 1 x 10
4
A308T
0.18 ± 0.02
0.16 ± 0.02
1.1 ± 0.3
150 ± 20
H318Y
0.046 ± 0.003
0.041 ± 0.002
2.0 ± 0.2
21 ± 2
Values represent the mean of four independent replicates ± standard deviation.