Table 1: Steady-state kinetic parameters of HsFH, A308T, and H318Y.

Enzyme Vmax (µmole /min/mg) kcat (s-1) Km (mM) kcat/Km (M-1 s-1)
HsFH 170 ± 10 150 ± 9 1.9 ± 0.2 8 x 104 ± 1 x 104
A308T 0.18 ± 0.02 0.16 ± 0.02 1.1 ± 0.3 150 ± 20
H318Y 0.046 ± 0.003 0.041 ± 0.002 2.0 ± 0.2 21 ± 2

Values represent the mean of four independent replicates ± standard deviation.