Fig. (10 A-H)
Immunocytochemical detection of phospho-Src in MCF-7 cells. Cells were incubated with 0.1 µL ml-1 DMSO (A & B), 1 µL ml-1 fish oil (C & D), 1 x 10-7 M 4-hydroxytamoxifen, 25 µM PD98059 and 5 µM LY294002 (E & F) or 1 µL mL-1 fish oil, 1 x 10-7 M 4-hydroxytamoxifen, 25 µM PD98059 and 5 µM LY294002 (G & H) for 3h. Cell were grown to 70% confluency on TESPA coated glass coverslips and fixed using formal saline. Primary antibody was Cell Signal Technology phoshpo-Src rabbit primary antibody at 1/40 for 2h. Secondary antibody was DAKO Rabbit EnVision peroxidase labeled polymer antibody for 2h. Magnification: A, C, E, G x 10 and B, D, F, H x 40, scale bar = 50μm. Passage numbers were 22, 27 and 33.