Fig. (3) Growth curves showing the growth rates of TamR cells when treated with PD98059, LY294002 and fish oil (both singly and in combination) and also Faslodex and estradiol. Cells were seeded at a density of 1.5 million cells per plate on day 0. Cells were counted on days 1, 2, 4, 7 and 9 and media and treatments were replenished on day 4. Cells were incubated at 37°C with 5% CO2. Cell counts represent the mean number per 3 counts (n=3 ± SD). Passage numbers were 25, 27 and 32. All test solutions significantly different to control (p 0.001).