Fig. (4) Migration assay showing the differences in the number of MCF-7, TamR and FasR cells migrating after 48h. Cells were seeded onto fibronectin-coated porous membranes at a density of 80,000 cells per well, incubated and allowed to migrate for 48h at 37°C with 5% CO2. No of cells migrated represents the mean number of cells per field of view (7 fields per insert were counted) (n=3 ± SD). Passage numbers were 12, 14, 20 for MCF-7 cells, 22, 25, 30 for TamR cells and 31, 35 and 40 for FasR cells.