Fig. (5) Migration assay demonstrating the significant reduction in the number of TamR cells migrating after 48h, when incubated with control, 1µL mL^-1 fish oil; and the significant potentiation of this effect when the cells were dosed with 1 μM L^-1 fish oil simultaneously with 25 µM PD98059 and 5 µM LY294002. Cells were seeded at a density of 80,000 cells per well and incubated at 37 °C with 5% CO₂ No of cells migrated represents the mean number of cells per field of view (7 fields per insert were counted) (n=3 ± SD). Passage numbers were 23, 27 and 30.