Table 2: Treatments for MCF-7 and TamR cells prior to Western blot and immunocytochemical analysis. PD98059 and LY294002 were made up in DMSO, while 4-hydroxytamoxifen was made up in ethanol. Treatments were incubated for 3 hours, after which protein was extracted for western blot assays and cells were fixed for ICC assays. Cells were maintained at 37°C with 5% CO2.

Cell line Treatment
MCF-7 0.1 µL mL-1 DMSO (Control)
1µL mL-1 Fish oil
1 x 10-7 M 4OHTam, 25µM PD98059, 5µM LY294002
1 µL mL-1 Fish oil, 1 x 10-7 M 4OHTam, 25µM PD98059, 5µM LY294002
TamR 0.1 µL mL-1 DMSO (Control)
1 µL mL20-1 Fish oil
25µM PD98059, 5µM LY294002
1 µL mL-1 Fish oil, 25µM PD98059, 5µM LY294002