Fig. (5) ELF MF exposure increases alkaline phosphatase protein levels. SaOS-2 cells were exposed or not to ELF MF for 1 h and after 0, 4, and 24 h, were lysed. ALP protein levels were determined by Western Blot analysis. Densitometric analysis of bands reveals about 40% increase of ALP expression at 0 h after exposure, which becomes about 150% at longer times. Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH) was used as normalization reference. Images shown are representative of three independent experiments.