Fig. (6) Alkaline phosphatase mRNA levels are not affected by ELF MF exposure. SaOS-2 cells were exposed or not to ELF MF for 1 h and after 0, 4, and 24 h, total mRNA was extracted. ALP mRNA levels were determined by Real-time PCR calculating ratios between 18S normalized signals from exposed and control cells. Reported data were expressed as means of three independent experiments ± standard deviations.