Fig. (4) An analysis of the plasma ACTH (A), β-endorphin (B), α-MSH (C), TGF-β (D), IgE (E), TNF-α (F), histamine (G), IL-4 (H), IL-10 (I), IFN-γ (J), IL-13 (K), and corticosterone (L) concentrations after the two-week treadmill treatment. In addition, the plasma corticosterone levels were used to investigate the circadian rhythm after the treadmill treatment (M). –AD: SPF NC/Nga mice, +AD: conventional NC/Nga mouse. The values are presented as the mean ± standard deviation, derived from 6 animals. *, P<0.05.