Fig. (5) The clock gene RNA follows circadian rhythms in the dorsal skin of mice. The circadian rhythm of the expression of Bmal1 RNA (A), Clock RNA (B), and Per2 RNA (C) was no longer observed after the two-week treadmill treatment. –AD: SPF NC/Nga mice, +AD: conventional NC/Nga mouse. The values are presented as the mean ± standard deviation, derived from 6 animals. The bar shows the pooled value and standard error of the mean.