Table 2: Effect of pyridoxine on metHb levels and lipid peroxidation in erythrocytes incubated with an oxidizing agent. The values correspond to means ± SD of experiments performed in triplicate. Erythrocytes were incubated 1 h with pyridoxine (1-100 μM) and an additional 10 hours with cumene hydroperoxide (100 μM). Control samples were not incubated with Hcum. The % metHb was calculated with reference to a positive control of 100% relative content (erythrocytes incubated with 0.4% potassium ferricyanide).

Samples Pyridoxine (µM) Hcum (µM) % metHb MDA (µM)
Control † 0 0 7.22 ± 0.31 4.44 ± 1.00
Hcum ¶ 0 100 9.32 ± 0.08 10.14 ± 0.83
Px1 ** 1 100 8.15 ± 0.08 8.18 ± 0.91
Px 10 ** 10 100 8.19 ± 0.05 7.96 ± 0.16
Px 100 ** 100 100 7.44 ± 0.15 7.69 ± 0.83
Control Px1 1 0 7.09 ± 0.17 3.94 ± 0.80
Control Px 10 10 0 7.01 ± 0.23 4.01 ± 0.73
Control Px 100 100 0 7.26 ± 0.01 4.24 ± 0.69

The difference between the values † vs ¶ y † vs ** was statistically significant, p < 0.05 (Student's t-test). Control: erythrocytes without any treatment. Control Px: erythrocytes only treated with pyridoxine, Px: Pyridoxine. HCum: Cumene Hydroperoxide. metHb: Methaemoglobin. MDA: Malondialdehyde.