Fig. (2) Purification of cysteine protease from Actinidia arguta using UNOsphere Q column chromatography.Fractions containing cysteine protease activity were collected. The bar indicates the fractions that were pooled. Protein concentration (mg/ml) (○), cysteine protease activity (■) and sodium chloride gradient (--). Insert: SDS-PAGE of fractions containing the enzyme activity. 3 μl of each aliquot (fraction numbers 37 to 45) was subjected to SDS-PAGE.