Table 3: Kinetic parameters of cysteine protease from A. arguta toward several substrates.

Substrate Km (μM) Vmax (μmoles/mg/min) Kcat (sec-1) Kcat/Km (sec-1μM-1)
Z-Leu-Arg-MCA 100 63.8 27.26 0.2726
Z-Val-Val-Arg-MCA 263 41.4 17.69 0.0672
Boc-Val-Leu-Lys-MCA 91 41.5 17.74 0.1949
Z-Phe-Arg-MCA 222 34.9 14.91 0.0671