Fig. (3) Interactions between rp eL42 and the Mdm2 protein as revealed by co-immunoprecipitation and western blotting. (A): immunoprecipitation of eL42 followed by the incubation with Mdm2, and vice versa, with (a): immunoblotting by means of anti-eL42 antibodies diluted 1/10.000. Input refers to the eL42 protein; (b): detection of the interaction between eL42 and Mdm2 with the anti-Mdm2 (D-12) antibodies diluted 1/1.000. Input refers to the Mdm2 protein; (B): As a control, the purified Mdm2 [1-350] protein was immunoprecipitated with anti-Mdm2 antibody (SMP14) and then incubated with p53. The interaction between the two proteins was detected by immunoblotting with anti-p53 (DO7) antibodies diluted to 1/500.000. I₁ refers to p53; M: protein molecular mass standards; SN: the supernatant after stopping the co-immunoprecipitation reaction; P: the pellet of immunocomplex containing the antibodies, the protein of interest and the antigen-associated proteins; I₂ refers to Mdm2.