Fig. (13) Effect of tetracycline on non-enzymatic A-site binding. Deacylated tRNAfMet and tRNAMet were used to occupy the P-site, while non-enzymatic A-site binding with AcPhe-tRNA binding to 70S (red line) or 80S (blue line) ribosomes, respectively was monitored. The 100% corresponds to the amount of Phe incorporated in the absence of antibiotic. The figure was reproduced from [24] with permission.