Table 10: Significance results of the minimum folding free energies of selected mammalian IREs.

Group	Name of IREs whose Folding Free Energies were Compared	Is the Energy Difference Significant (p < 0.05)?
5’IREs containing a UGC/C internal loop	FTH 5’IRE vs FTL 5’IRE	Yes
5’IREs containing a C bulge	ACO2 5’IRE vs. FPN 5’IRE	Yes
	ACO2 5’IRE vs. ALAS2 5’IRE	Yes
	ACO2 5’IRE vs. HIF2α 5’IRE	N/A
	FPN 5’IRE vs. ALAS2 5’IRE	Yes
	FPN 5’IRE vs. HIF2α 5’IRE	N/A
	ALAS2 5’IRE vs. HIF2α 5’IRE	N/A
5’IRE containing a UGC/C internal loop vs. 5’IRE containing a C bulge	FTH 5’IRE vs. ACO2 5’IRE	Yes
	FTH 5’IRE vs. FPN 5’IRE	Yes
	FTH 5’IRE vs. ALAS2 5’IRE	No
	FTH 5’IRE vs. HIF2α 5’IRE	N/A
	FTL 5’IRE vs. ACO2 5’IRE	Yes
	FTL 5’IRE vs. FPN 5’IRE	Yes
	FTL 5’IRE vs. ALAS2 5’IRE	Yes
	FTL 5’IRE vs. HIF2α 5’IRE	N/A
Pooled 5’IREs containing a UGC/C internal loop vs. Pooled 5’IREs containing a C bulge		No
TFRC 3’IREs	1st TFRC 3’IRE vs. 2nd TFRC 3’IRE	No
	1st TFRC 3’IRE vs. 3rd TFRC 3’IRE	Yes
	2nd TFRC 3’IRE vs. 3rd TFRC 3’IRE	Yes
3’IREs	CDC14A vs. DMT1	No
	CDC14A vs. 1st TFRC 3’IRE	No
	CDC14A vs. 2nd TFRC 3’IRE	No
	CDC14A vs. 3rd TFRC 3’IRE	Yes
	CDC14A vs. all of the TFRC 3’IREs	Yes
	DMT1 vs. 1st TFRC 3’IRE	No
	DMT1 vs. 2nd TFRC 3’IRE	No
	DMT1 vs. 3rd TFRC 3’IRE	Yes
	DMT1 vs. all of the TFRC 3’IREs	Yes
Pooled 5’IREs vs. Pooled 3’IREs		No