Fig. (1). Purified proteins used in this study Aliquots (0.5 μg) of each of the purified TaCdc6 proteins were fractionated on a 12% SDS-PAGE gel and visualized by Coomassie blue staining. Lane 1: molecular mass marker; lane 2, T. acidophilum Cdc6-1; lane 3, T. acidophilum Cdc6-2.