Fig. (3). T. acidophilum Cdc6-1 and -2 is abundantly expressed in vivo during exponential growth phase A.Growth curve of T. acidophilum. The number of cells (108) and density (OD600) are plotted versus time (hours). The arrow indicates the time of harvesting.BQuantitative Western blot of TaCdc6-1 inT. acidophilumwas performed as described in “Materials and Methods”. T. acidophilumextract corresponding to a certain number of cells (x 107) and amount of purified, recombinant TaCdc6-1 protein (fmol) loaded onto each lane are indicated.C Quantitation of TaCdc6-2 in T. acidophilum cells was performed as described in B.