Fig. (4). The autophosphorylation activity of T. acidophilum Cdc6 is not affected by DNA or MCM A.The activity of TaCdc6 is not affected by DNA. Cdc6 autophosphorylation reactions were performed as described in “Materials and Methods”in a reaction mixture (15 μl) containing 10 pmol Cdc6 protein in the absence (lane 2 and 5) or in the presence of 1 μg ssDNA (lane 3 and 6) or 1 μg dsDNA ((lane 4 and 7). Upper panel shows a representative 12% Coomassie stained SDS-polyacrylamide gel. Lower panel shows a representative autoradiogram.B.Cdc6 autophosphorylation reactions were performed as described in “Materials and Methods” in a reaction mixture (15 μl) containing 10 pmol Cdc6 protein in the absence (lane 3 and 5) or presence of 20 pmol MCM (lane 4 and 6). Upper panel shows a representative 12% Coomassie stained SDS-polyacrylamide gel. Lower panel shows a representative autoradiogram.