Fig. (5) Western blot analysis of 10¹² phage particles (quantified by A₂₆₀ measurements) prepared 10 h after helper phage infection and detection by polyclonal anti-PhoA rabbit antibody ab7319. Lane 1: 7.81∙10^-5 mg wtPhoA (= 10¹² PhoA subunits). Lane 2: PhoA-pIII-phagemid amplified without IPTG. Lane 3: PhoA-pIII-phagemid amplified in the presence of 1 mM IPTG. Lane 4: PhoA-pVIII-phagemid amplified without IPTG. Lane 3: PhoA-pVIII-phagemid amplified in the presence of 1 mM IPTG.