Table 1: Michaelis Constants of Different Phagemid Prepara-tions and of Soluble PhoA

Sample c(IPTG) [µM] Km [µM]d
pIII-PhoA 0 11.2 (± 0.1)
pIII-PhoA 1 12.3 (± 0.9 )
pIII-PhoA 10 9.0 (± 0.8 )
pIII-PhoA 100 9.8 (± 1.1 )
pIII-PhoA 1000 10.1 (± 1.2 )
pVIII-PhoA 0 12.9 (± 1.0 )
pVIII-PhoA 1 12.9 (± 1.2 )
pVIII-PhoA 10 12.3 (± 0.9)
pVIII-PhoA 100 11.0 (± 0.7)
pVIII-PhoA 1000 11.0 (± 0.6)
PhoAa 12.3 (± 0.2)
PhoAb 12.7
PhoAc 8.5

a) Km from the present work
b) Km from [ 24]
c) Km from [ 23]
d) The standard deviation derived from three experiments performed with independent freshly prepared phagemids is given in parentheses.