Table 1:
Heparin affinity and uPA inhibition of native and heated Serp-1 Proteins
Samples
Proportion
*
(%)
Heparin Affinity
**
(mM NaCl)
uPA Inhibition
Native Serp-1
Unbound
5
0
No
Peak 1
95
555
Yes
Heated Serp-1
Unbound
6
0
No
Peak 1
86
400
No
Peak 2
8
680
Yes
*
Protein concentration was measured using the Bio-Rad Protein Assay Kit
**
Heparin affinity is given as the salt concentration required for elution
***
Measured in uPA activity assay