Fig. (10) Alignment of amino acid sequences of IleRS from different origins, around the identified labeled residues of E. coli IleRS.The labeled amino acid residues covalently attached to the reactive substrate analogs used in this study are numbered (in bold) according to the numbering of the corrected IleRS amino acid sequence from E. coli reported here. Alignment of amino acid sequences of IleRS from Staphylococcus aureus (Sa), Thermus thermophilus (Tt) and Homo sapiens (Hs) with that of the same synthetase from E. coli around the identified labeled residues was achieved with CLUSTAL. Asteriks indicate identical residues, double dots and single dots indicate respectively highly and weakly conserved residues.