Fig. (7) Saturation of IleRS by ADP-PL.The incubation mixtures were the same as in Figure 5. The fraction of inactivated IleRS, as deduced from kinetics data from Figure 5, is represented as a function of free ADP-PL concentration. From this saturation curve, the half-effect concentration of ADP-PL in the inactivation of IleRS was estimated to be 0.17 mM.