Table 2: Experimental Ф-Values Obtained from the Unfolding of Protein G by Denaturant and Theoretical Ф-Values Calculated from the Modeling of Protein G Unfolding under the External Forces, Correlation Coefficients Between Theoretical and Experimental Ф-Values for the Ensemble of Transition State Structures for Protein G

Mutation	Ф- values
	Experimental	Theoretical
		v=0.125 Å·ps-1		v=0.0625 Å·ps-1
		First Peak	Second Peak	First Peak	Second Peak	Third Peak
I6A	0.38	0.61	0.22	0.63	0.22	0.22
L7A	0.32	0.55	0.3	0.55	0.34	0.23
T11A	0.02	0.21	0.02	0.23	0.02	0.1
T16A	0.00	0.69	0.58	0.71	0.56	0.53
A20G	0.02	0.58	0.41	0.59	0.33	0.26
D22A	0.23	0.84	0.73	0.84	0.68	0.39
A26G	0.31	1	0.61	0.77	0.51	0.3
V29A	0.26	0.64	0.64	0.68	0.46	0.34
K31G	0.23	0.64	0.39	0.64	0.37	0.16
Q32G	0.55	0.72	0.68	0.7	0.55	0.31
Y33A	0.20	0.69	0.58	0.68	0.49	0.41
A34G	0.21	0.67	0.55	0.66	0.51	0.27
N35G	0.19	0.75	0.65	0.74	0.63	0.27
V39A	0.16	0.52	0.33	0.54	0.3	0.22
D46A	0.96	0.8	0.82	0.84	0.79	0.84
D47A	0.67	0.77	0.73	0.65	0.68	0.74
T49A	0.84	0.79	0.8	0.83	0.79	0.82
T51A	0.44	0.75	0.35	0.75	0.31	0.33
T53A	0.27	0.57	0.33	0.59	0.33	0.34
V54A	0.16	0.59	0.28	0.59	0.27	0.16
correlation coefficients		0.48	0.57	0.53	0.62	0.76