Table 2: EC50 and Emax Values of Anandamide Analogues for Stimulating [35S]GTPγS Binding in Rat Microsomal Membranes a
CB1 Analogues
EC50 (µM)
Emax (%)
A
0.48 ± 0.03
112 ± 4.8
B
0.22 ± 0.07
121 ± 1.5
C
0.34 ± 0.08
133 ± 3.5
D
0.52 ± 0.05
108 ± 2.7
E
0.42 ± 0.03
103 ± 1.9
F
0.27 ± 0.04
127 ± 4.5
WIN 55,212-2
0.18 ± 0.09
144 ± 6.5
CP-55,940
0.10 ± 0.05
135 ± 9.0
a[35S]GTPγ-S binding assay was conducted using eight concentrations of each analogue being tested and two experiments run with four duplicates of each point.