Molecules Acting on CB1 Receptor and their Effects on Morphine Withdrawal In Vitro

Table 4.: The Effect of CB1Rs Agonist WIN 55,212-2, CP 55,940 or A-F [(1) 1×10^-7-(2) 5×10^-8- (3) 1×10^-8 M] on Electrical Stimulation and Final Opioid Withdrawal

Compounds	Electrical Stimulation	Final Morphine Withdrawal

WIN 55,212-2	75.6±3.9^** 1	62.5±5.3^** 1
	46.8±2.7^** 2	39.7±3.1^* 3
	35.4±1.6^** 3	22.4±2.3 3

CP 55,940	82.3±4.8^** 1	57.3±2.5^** 1
	56.3±2.4^** 2	44.8±4.9^* 2
	37.3±2.7 3	35.3±2.4 3

CB1R-A	72.8±2.5^** 1	72.3±3.7^** 1
	49.7±2.9^** 2	57.2±3.6^** 2
	33.2±3.6 3	41.5±3.2^* 3

CB1R-B	77.5±4.2^** 1	69.5±4.6^** 1
	59.3±3.6^** 2	42.5±2.3^** 2
	23.7±2.5 3	28.7±3.9 3

CB1R-C	69.3±5.8^** 1	68.7±5.1^** 1
	48.4±3.2^** 2	44.3±2.1^** 2
	32.3±2.1 3	20.7.6±1.9 3

CB1R-D	73.1±6.7^** 1	70.2±6.2^** 1
	55.1±3.2^** 2	48.2±3.3^** 2
	38.3±2.5 3	27.3±3.1 3

CB1R-E	67.3±6.1^** 1	73.4±4.3^** 1
	46.9±2.9^** 2	62.5±2.5^** 2
	36.2±6.5 3	44.3±5.8 3

CB1R-F	80.1±5.7^** 1	79.2±6.1^** 1
	63.9±3.5^** 2	59.3±3.1^** 2
	49.2±3.9^** 3	46.2±2.2^** 3

Results are expressed as % of inhibition (mean±s.e.m.);

^* P<0.05;

^** P<0.01. All data were compared to the pre-drug.