Fig. (3). Ubiquitinated protein binding to HRS does not affect the association between HRS and ARMc8α, and exogenously expressed ARMc8α increases the interaction of ubiquitinated proteins with exogenously expressed HRS via the UIM A. In CHO cells, pFLAG-ARMc8α was co-transfected with pT7-HRS, pT7-HRS (S270E), or pT7-HRSΔUIIM, as indicated (lanes 2 to 4). Exogenous ARMc8α was immunoprecipitated with anti-FLAG antibody. Co-immunoprecipitations of each deleted HRS and ubiquitin were analyzed by immunoblotting with the antibodies indicated. As a negative control, cells were co-transfected with pFLAG-Mock and pT7-HRS (lane 1). Arrow indicates the location of the lane beginning.B. In CHO cells, pFLAG-ARMc8α or pFLAG-Mock was co-transfected with pT7-HRS or pT7-HRSΔUIM, as indicated (lanes 1 to 3). The transfected cells were lysed and the exogenous HRS and each deleted HRS were immunoprecipitated with T7•tag antibody. This was followed by immunoblotting with the antibodies shown (lanes 4 to 6). IB, immunoblot. IP, immunoprecipitation.