Fig. (1) Effects of various detergents on the activity of LipA (○) and LipB (□). Increasing concentrations of the following detergents were used; SDS (A), Na cholate (B), CTAB (C), Triton X-100 (D), Tween 20 (E) and Tween 80 (F). Assays were carried out as described in the materials and methods section with some modifications. The assay buffer was 100 mM TAPS adjusted at pH25°C9.0. The assay temperature was 92°C. Non ionic detergents at concentrations higher than their CMC values caused turbidity in the assay solution, thus, assays were centrifuged and diluted before measuring the absorption.