Effects of Detergents on Activity, Thermostability and Aggregation of Two Alkalithermophilic Lipases from Thermosyntropha lipolytica

Table 2: The Effect of E600 on LipA and LipB Activity in the Presence of SDS and Tween 20

[SDS]	LipA^a (U•mg^-1)	LipA+E600 (U•mg^-1)	LipB^b (U•mg^-1)	LipB+E600 (U•mg^-1)
0	11.5 ± 0.6	8.6 ±1.0	11.8 ± 0.6	9.0 ± 1.0
1mM	7.6 ± 1.0	6.0 ± 1.0	9.0 ± 1.0	6.2 ± 1.0
3 mM	7.8 ± 2.0	2.0 ± 1.0	8.4 ± 2.0	2.3 ± 1.0
7mM	8.0 ± 2.0	3.0 ± 1.4	8.5 ± 2.0	1.6 ± 1.4
[Tween 20]	LipA (U·mg^-1)	LipA+E600 (U·mg^-1)	LipB (U·mg^-1)	LipB+E600 (U·mg^-1)
0	11.6 ± 0.5	9.6 ± 1.0	12.4 ± 0.5	9.0 ± 1.0
0.008 mM	10.0 ± 1.2	5.0 ± 1.4	9.5 ±1.6	5.4 ± 1.5
0.01 mM	9.0 ± 2.0	3.0 ± 2.0	9.2 ± 2.0	1.0 ± 0.5
0.04 mM	7.6 ±1.2	3.4 ± 1.6	7.0 ± 1.4	1.8 ±1.4
0.08 mM	7.0 ± 1.0	2.4 ± 1.0	6.8 ± 1.4	3.5 ±1.6
1.0 mM	6.4 ± 1.8	3.0 ± 1.4	6.5 ± 1.8	3.8 ± 1.4

^a In the absence of detergents, LipA and LipB retained 90% activity after incubation at 60°C for 3 h.

^b LipA and LipB specific activities before incubation were 12.4 and 13.0 U.mg^-1, respectively.