Table 2: The Effect of E600 on LipA and LipB Activity in the Presence of SDS and Tween 20

[SDS] LipAa (U•mg-1) LipA+E600 (U•mg-1) LipBb (U•mg-1) LipB+E600 (U•mg-1)
0 11.5 ± 0.6 8.6 ±1.0 11.8 ± 0.6 9.0 ± 1.0
1mM 7.6 ± 1.0 6.0 ± 1.0 9.0 ± 1.0 6.2 ± 1.0
3 mM 7.8 ± 2.0 2.0 ± 1.0 8.4 ± 2.0 2.3 ± 1.0
7mM 8.0 ± 2.0 3.0 ± 1.4 8.5 ± 2.0 1.6 ± 1.4
[Tween 20] LipA (U·mg-1) LipA+E600 (U·mg-1) LipB (U·mg-1) LipB+E600 (U·mg-1)
0 11.6 ± 0.5 9.6 ± 1.0 12.4 ± 0.5 9.0 ± 1.0
0.008 mM 10.0 ± 1.2 5.0 ± 1.4 9.5 ±1.6 5.4 ± 1.5
0.01 mM 9.0 ± 2.0 3.0 ± 2.0 9.2 ± 2.0 1.0 ± 0.5
0.04 mM 7.6 ±1.2 3.4 ± 1.6 7.0 ± 1.4 1.8 ±1.4
0.08 mM 7.0 ± 1.0 2.4 ± 1.0 6.8 ± 1.4 3.5 ±1.6
1.0 mM 6.4 ± 1.8 3.0 ± 1.4 6.5 ± 1.8 3.8 ± 1.4

a In the absence of detergents, LipA and LipB retained 90% activity after incubation at 60°C for 3 h.
b LipA and LipB specific activities before incubation were 12.4 and 13.0 U.mg-1, respectively.