Fig. (3). AEDANS Fluorescence Quenching by Acrylamide 2 µM AEDANS-CaBD in the presence of 100 µM EDTA (square), 2 mM Mg²⁺ plus 1 mM EGTA (triangle), or 100 µM Ca²⁺ (circle) was titrated with increasing amounts of acrylamide. The standard Stern-Volmer equation was used to fit the data as described in the experimental section and their K_sv values are shown in Table 1.