Fig. (5). Kinetics of Metal Dissociation Ca²⁺-bound protein was quick-mixed with a solution containing large excess of EDTA and its fluorescent decay at 460 nm was recorded with the excitation wavelengths of 340 nm. (A) Shows the kinetic trace of Ca²⁺ dissociation from AEDANS-E99Q/E143Q, where (B) and (C) show the kinetics of Ca²⁺ and Mg²⁺ dissociation from AEDANS-CaBD. The traces are representative of four to six independent experiments each.