Table 1:
Stern-Volmer Quenching Constants Obtained from Acrylamide Titration
K
sv
(M
-1
)
Protein
apo
Mg
2+
Ca
2+
CaBD
a
5.28 ± 0.50
5.04 ± 0.29
9.49 ± 0.41
E99Q/E143Q
a
3.01 ± 0.25
2.92 ± 0.19
2.95 ± 0.11
AEDANS-CaBD
b
3.02 ± 0.42
6.40 ± 0.82
12.01 ± 1.025
AEDANS-E99Q/E143Q
b
2.10 ± 0.21
2.46 ± 0.32
2.97 ± 0.26
a
The data were obtained by fitting with a linear Stern-Volmer equation.
b
The labeled proteins were excited at 340 nm and their intensities at the maximum emission wavelength were recorded.