Fig. (2) Intracellular localization of ASR1 in yeast cells. Wild type cells (YPH102 strain) (panels A1-E3) and hog1Δ (JBY13 strain) (F1-I3 panels) transformed with pYES2-Asr1 were incubated in 1% galactose SC medium. After 20 minutes, samples were withdrawn (panels A, E and F) and remaining cells were transferred to 2% glucose fresh SC medium with (panels B, C, G and H) or without (panels D and I) 0.5 M NaCl. Samples were withdrawn at 10 minutes (panels B and G) or 20 minutes (panels C, D, H and I). Nuclei were stained with propidium iodide (panels 1). Cells were processed for indirect immunofluorescence with ASR1-specific antiserum (panels 2), except for panels E where a pre-immune serum was used. Merged images are shown in panels 3.