Fig. (2) Proliferation in JEG-3 cells. Placental cells were exposed to nicotine in absence of antioxidant nutrient and in presence of vitamin C, vitamin E and NAC, alone (A) or in combination (B). Cell growh was estimated after 24 h by MTT method. Results are expressed as mean ± SD of six experiments, each one performed in triplicate. ** P< 0.01.